Exogenous melatonin improves germination rate in buckwheat under high temperature stress by regulating seed physiological and biochemical characteristics.

The germinations of three common buckwheat (Fagopyrum esculentum) varieties and two Tartary buckwheat (Fagopyrum tataricum) varieties seeds are known to be affected by high temperature. However, little is known about the physiological mechanism affecting germination and the effect of melatonin (MT) on buckwheat seed germination under high temperature. This work studied the effects of exogenous MT on buckwheat seed germination under high temperature. MT was sprayed. The parameters, including growth, and physiological factors, were examined. The results showed that exogenous MT significantly increased the germination rate (GR), germination potential (GP), radicle length (RL), and fresh weight (FW) of these buckwheat seeds under high-temperature stress and enhanced the content of osmotic adjustment substances and enzyme activity. Comprehensive analysis revealed that under high-temperature stress during germination, antioxidant enzymes play a predominant role, while osmotic adjustment substances work synergistically to reduce the extent of damage to the membrane structure, serving as the primary key indicators for studying high-temperature resistance. Consequently, our results showed that MT had a positive protective effect on buckwheat seeds exposed to high temperature stress, providing a theoretical basis for improving the ability to adapt to high temperature environments.

Reproducibility in nontarget screening (NTS) of environmental emerging contaminants: Assessing different HLB SPE cartridges and instruments.

Non-targeted screening (NTS) methods are integral in environmental research for detecting emerging contaminants. However, their efficacy can be influenced by variations in hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) cartridges and high-resolution mass spectrometry (HRMS) instruments across different laboratories. In this study, we scrutinized the influence of five HLB SPE cartridges (Nano, Weiqi, CNW, Waters, and J&K) and four LC-HRMS platforms (Agilent, Waters, Thermo, and AB SCIEX) on the identification of emerging environmental contaminants. Our results demonstrate that 87.6 % of the target compounds and over 59.6 % of the non-target features were consistently detected across all tested HLB cartridges, with an overall 71.2 % universally identified across the four LC-HRMS systems. Discrepancies in detection rates were primarily attributable to variations in retention time stability, mass stability of precursors and fragments, system cleanliness affecting fold change and p-values, and fragment response. These findings confirm the necessity of refining parameter criteria for NTS. Moreover, our study confirms the efficacy of the PyHRMS tool in analyzing and processing data from multiple instrumental platforms, reinforcing its utility for multi-platform NTS. Overall, our findings underscore the reliability and robustness of NTS methods in identifying potential water contaminants, while also highlighting factors that may influence these outcomes.

Dynamic composting actuated by a Caldibacillus thermoamylovorans isolate enables biodecomposability and reusability of Cinnamomum camphora garden wastes.

The ecotoxic substances in Cinnamomum camphora garden wastes (CGW) often restrain microbe-driven composting process. Here, a dynamic CGW-Kitchen waste composting system actuated by a wild-type Caldibacillus thermoamylovorans isolate (MB12B) with distinctive CGW-decomposable and lignocellulose-degradative activities was reported. An initial inoculation of MB12B optimized for temperature promotion with reduced emission of CH4 and NH3 by 61.9% and 37.6%, respectively, increased germination index and humus content by 18.0% and 44.1%, respectively, and reduced moisture and electrical conductivity, and all were further reinforced by reinoculation of MB12B during the cooling stage of composting. High-throughput sequencing showed varied bacterial community structure and abundance following MB12B inoculation, with temperature-relative Caldibacillus, Bacillus, and Ureibacillus, and humus-forming Sphingobacterium emerging to dominate abundance, which strongly contrasted with Lactobacillus (acidogens related to CH4 emission). Finally, the ryegrass pot experiments showed significant growth-promoting effectiveness of the composted product that successfully demonstrated the decomposability and reuse of CGW.

Insights into the accumulation, distribution and toxicity of pyrene associated with microplastics in rice (Oryza sativa L.) seedlings.

Microplastic and polycyclic aromatic hydrocarbons (PAHs) can be introduced into agroecosystems through various agricultural activities and may threaten food safety and human health. However, little research has focused on the behavior of microplastics-associated PAHs and their toxicity effects in agroecosystems, especially in crops. In the present study, we investigated the accumulation, distribution and toxicity of pyrene associated with polyethylene (PE) microplastics in rice (Oryza sativa L.). With quantitative analysis using 14C isotope labelling, the total accumulation efficiency of 14C-pyrene in rice seedlings was 22.4 ± 1.2% and 14.5 ± 0.3% when exposed to freely dissolved pyrene and PE-associated pyrene, respectively. The translocation of 14C-pyrene was significantly decreased by microplastics adsorption even when the amount of pyrene in the rice roots had no significant difference. Subcellular distribution of 14C-pyrene in rice suggested that PE microplastics-associated pyrene located more on cell walls than free dissolved pyrene. Furthermore, results showed free pyrene, but not PE-associated pyrene, significantly decreased the length and biomass of rice roots as well as increased the activities of antioxidant enzymes (superoxide dismutase and catalase). It indicated that the association with microplastics alleviated the phytotoxicity of pyrene in rice seedlings. These findings shed new light on the environmental behavior and effects of PAHs associated with microplastics in crops and will be helpful to its comprehensive risks assessment.

Photodissolution of submillimeter-sized microplastics and its dependences on temperature and light composition.

Photodissolution has the potential to efficiently remove microplastics from the surface ocean. Here, we examined the effects of temperature and incident sunlight composition on the photodissolution of submillimeter-sized microplastics of polypropylene (PP), polystyrene (PS), and thermoplastic polyurethane (TPU) in seawater. The photoproduction of dissolved organic carbon (DOC), chromophoric dissolved organic matter, and dissolved nitrogen (TPU only) was observed to increase exponentially within 7 days of full-spectrum irradiation. The temperature dependence of photodissolution increased with irradiation time for PP and PS but remained relatively constant for TPU. A 20 °C increase in temperature enhanced DOC photoproduction by 10 times for PP, three times for PS, and four times for TPU at 7-d irradiation, giving activation energies of 59.4-84.8 kJ mol-1. Photodissolution of all three polymers was exclusively driven by ultraviolet-B (UVB) radiation. PS-derived DOC was photomineralizable, while PP- and TPU-derived DOC appeared photo-resistant. Extrapolating the lab-based DOC photoproduction rates to warm surface oceans yields lifetimes of 6.5 years for PP, 3.6 years for PS, and 3.7 years for TPU. This study demonstrates that photodissolution of the tested microplastics is restricted to the thin UVB-penetrable surface ocean and that water temperature plays a critical role in controlling the photodissolution of these microplastics.

Effect of body weight on bleeding events of aspirin in ischemic stroke or transient ischemic attack patients.

WHAT IS KNOWN AND OBJECTIVES: Bleeding is the most common adverse reaction to aspirin and can lead to drug discontinuation or even be life-threatening in the secondary prevention of stroke or transient ischemic attack. The aim of this study was to evaluate risk factors for bleeding adverse reaction of aspirin in ischemic stroke or transient ischemic attack. METHODS: This retrospective analysis included patients treated with aspirin (100 mg) as a secondary prevention for ischemic stroke or transient ischemic attack. The bleeding events that occurred during the first year were collected, including gastrointestinal, skin, nasal cavity, gum, and urinary tract bleeding events. Then, univariate and multivariate logistic regression analyses were used to identify independent factors associated with bleeding events of aspirin. RESULTS AND DISCUSSION: A total of 578 patients were enrolled in this study, and 58 patients developed bleeding during the first year of secondary prevention. Body weight and combination with selective serotonin reuptake inhibitors were found to be significant risk factors for overall bleeding (p = 0.025 and 0.012). Body weight below 60 kg was a risk factor for overall bleeding and gastrointestinal bleeding events. WHAT IS NEW AND CONCLUSION: Patients weighing less than 60 kg were at increased risk of bleeding with 100 mg aspirin for secondary prevention of cerebral infarction transient ischemic attack.

Effects of nano- and microplastics on the bioaccumulation and distribution of phenanthrene in the soil feeding earthworm Metaphire guillelmi.

Microplastics (MPs) and nanoplastics (NPs), are collectively referred to as fine plastic particles (FPs), have been reported for both the "vector" effect and "dilution" effect which alters the bioaccumulation of organic contaminants. However, which effect plays a dominant role, especially in terrestrial ecosystems, remains unknown. In the present study, we used 14C-radioactive labeling tracing technique to assess the sorption of a typical polycyclic aromatic hydrocarbon, phenanthrene on soil particles and FPs, as well as the contribution of vector effects of FPs on the bioaccumulation and distribution of phenanthrene by the geophagous earthworm Metaphire guillelmi. The results showed that the presence of FPs in soil decreased the bioaccumulation of 14C-Phenanthrene in M. guillelmi by decreasing the bioavailable fraction of phenanthrene in soil, and the decreasing effect was more dramatic for NPs treatments. In all cases, bioaccumulation of 14C-Phenanthrene in M. guillelmi was still determined by the free concentration of 14C-Phenanthrene in soil and limited vector effects was observed. Moreover, the different correlation coefficients between the free concentration of 14C-Phe in two soils and bioaccumulated 14C-Phenanthrene in earthworms indicated that soil properties remained a dominant factor that determines the bioaccumulation efficiency of 14C-Phenanthrene in the FPs-soil system. Although the total 14C-Phenanthrene bioaccumulation in earthworms did not increase, vector effects may be responsible for the increased relative distribution of 14C-phenanthrene in the organ region, compared with skin and gut regions, leading to unknown risks to organs that are sensitive to these contaminants.

Nomogram Model for Predicting the Risk of Multidrug-Resistant Bacteria Infection in Diabetic Foot Patients.

OBJECTIVE: This study established an individualized nomogram for predicting the risk of multidrug-resistant bacterial (MDRB) infection in patients with the diabetic foot (DF), and providing a reference for clinical prevention and treatment. METHODS: A total of 199 DF patients admitted to the hospital from July 2015 to December 2018 were included in this study. The pathogenic bacteria at the site of infection were detected and the factors affecting the occurrence of MDRB infection in DF patients summarized. The R software was used to draw the nomogram, and the Bootstrap Method used to internally verify the model. The calibration curve and the Harrell's Concordance Index (C-index) were used to evaluate the predictive effect of the nomogram model. RESULTS: Logistic regression analysis showed that age, course of diabetes, previous use of antibacterial drugs, types of antibacterial drugs, and osteoporosis were risk factors for multidrug-resistant infections in DF (P<0.05). The area under the receiver operating characteristic curve (AUC, Area Under Curve) of the nomogram model after internal verification was 0.773 (95% CI: 0.704-0.830). The mean absolute error between the predicted probability of infection in the nomogram and the actual occurrence of MDRB was 0.032, indicating that the nomogram model had good forecasting efficiency and stability. CONCLUSION: The risk factors for multidrug-resistant infections in DF are age, course of diabetes, previous use of antibacterial drugs, types of antibacterial drugs used, and osteoporosis. The nomogram model drawn on these risk factors has good predictive accuracy and can assist medical staff in formulating targeted infection prevention strategies for patients.

Quantification of polystyrene plastics degradation using 14C isotope tracer technique.

14C-isotope tracer technique is an effective tool for quantitative analysis of compounds. Based on its unique 14C signal, 14C-isotope tracer has been widely used in degradation of pollutants, especially in a complex environmental matrix. In this chapter, we introduce methods and examples for studying the degradation of polystyrene (PS) plastics using the 14C-isotope tracer technique. 14C-based gel permeation chromatography (GPC), high performance liquid chromatography (HPLC) as well as liquid scintillation counter (LSC) are introduced for analyzing changes in the molecular weight of plastic polymers, generation of hydrophilic products, and complete mineralization of plastics during microbial and UV-induced degradation. With the 14C-isotope tracer technique, further studies on mechanisms for degradation of plastics/microplastics under complex natural environment conditions could be facilitated.

Aging Processes of Polyethylene Mulch Films and Preparation of Microplastics with Environmental Characteristics.

In this study, we explored the aging processes of a commercial polyethylene (PE) mulch film under UV irradiation and compared the laboratory aged films with films aged in nature. Overall, the aged films obtained from laboratory conditions were similar with that from natural conditions. Among the investigated factors, UV irradiation was crucial in the aging of the films, producing cracks and oxygen-containing functional groups on the films surface, constantly with natural aging. The formation of cracks induced a decrease of mechanical strength as well as the formation of MPs on the surface. The chemical oxidations detected by Fourier-transform infrared spectroscopy (FTIR) usually happened after the observed physical changes during aging. Moreover, a protocol was developed for laboratory preparation of MPs with characteristics similar with that from environmental aging and PE MPs with sizes of 2-400 µm could be produced in large amounts at relatively short period of time.

MiRNA-145 and Its Direct Downstream Targets in Digestive System Cancers: A Promising Therapeutic Target.

MicroRNAs (miRNAs) play a vital role in the onset and development of many diseases, including cancers. Emerging evidence shows that numerous miRNAs have the potential to be used as diagnostic biomarkers for cancers, and miRNA-based therapy may be a promising therapy for the treatment of malignant neoplasm. MicroRNA-145 (miR-145) has been considered to play certain roles in various cellular processes, such as proliferation, differentiation and apoptosis, via modulating the expression of direct target genes. Recent reports show that miR-145 participates in the progression of digestive system cancers, and plays crucial and novel roles in cancer treatment. In this review, we summarize the recent knowledge concerning the function of miR-145 and its direct targets in digestive system cancers. We discuss the potential role of miR-145 as a valuable biomarker for digestive system cancers and how miR-145 regulates these digestive system cancers via different targets to explore the potential strategy of targeting miR-145.

Interactions between microplastics and organic pollutants: Effects on toxicity, bioaccumulation, degradation, and transport.

Microplastics (MPs), defined as particles with diameters <5 mm and including nanoplastics (NPs), with diameters <1 µm, are characterized by large specific surface areas and hydrophobicity. In aquatic and terrestrial environments, MPs interact with co-occurring organic pollutants through sorption and desorption, which alters the environmental behavior of the pollutants, such as their toxicity, bioaccumulation, degradation, and transport. In this review, we summarize the results of current studies of the interactions between MPs and organic contaminants, and focus on the different mechanisms and subsequent ecological risks of contaminant transfer among environmental media, MPs and organisms. The sorption/desorption of organic pollutants on/from MPs is discussed with respect to solution conditions and the properties of both the MPs and the pollutants. More importantly, the ability of MPs to alter the toxicity, bioaccumulation, degradation, and transport of organic pollutants through these interactions is considered as well. We then examine the interrelationships of the different environmental behaviors of MPs and organic pollutants and the roles played by environmental processes. Finally, we identify the remaining knowledge gaps that must be filled in further studies in order to accurately evaluate the environmental risks of MPs and their associated organic pollutants.

Fate of bisphenol S (BPS) and characterization of non-extractable residues in soil: Insights into persistence of BPS.

The environmental fate and persistence of bisphenol S (BPS), a substitute for bisphenol A (BPA), are unclear. This study used 14C-labeled BPS to examine the fate, biodegradation, and residue properties of BPS incubated in an oxic soil for 28 days. BPS dissipated quickly, with a half-life of 2.8 days. Most of the BPS was mineralized (53.6 ± 0.2% of initial amount by day 28) or transformed into non-extractable residues (NERs) (45.1 ± 0.3%), with generation of minor extractable residues (3.7 ± 0.2%) containing two metabolites. NERs were formed mainly via physico-chemical entrapment (51.1 ± 2.4% of the total NERs, consisting almost exclusively of BPS) and ester-linkages (31.5 ± 3.0% of the total NERs, consisting of both BPS and polar metabolites). When mixed with fresh soil, BPS-derived NERs became unstable and bioavailable. Subsequent mineralization was determined for 19.5 ± 1.1% of the total NERs and 35.5 ± 2.6% of the physico-chemically entrapped BPS. A fate model was used to describe the kinetics of NER formation, which indicated that microbial activity in soil could have strongly reduced the kinetic rate of the release of physico-chemically entrapped NERs into free form and therefore increased the stability of this type of NERs in soil. Our results provide unique insights into the fate of BPS in soil and suggest that while BPS is biodegradable, it includes the formation of large amounts of reversibly physico-chemically entrapped and covalently bound ester-linked NERs. The instability of these NERs should be considered in assessments on environmental persistence and risks of BPS. Our study also points out the environmental importance of NERs of agrochemicals.

Biodegradation of bisphenol compounds in the surface water of Taihu Lake and the effect of humic acids.

Bisphenol analogues (BPs) pollution in the aquatic environment is increasingly a worldwide concern. There is an urgent need to understand the fate of BPs in the aquatic environment. In this study, we studied the biodegradation of eight BPs in Taihu Lake and discussed the effect of humic acid (HA), which was extracted from Taihu Lake sediment, on the disappearance of BPs. Under aerobic conditions, bisphenol AF (BPAF) and bisphenol S (BPS) were recalcitrant to biodegradation in the lake water. The half-lives for bisphenol F (BPF), bisphenol A (BPA), bisphenol B (BPB), bisphenol E (BPE), bisphenol Z (BPZ), and bisphenol M (BPM) ranged from 34 to 75 days in the Taihu Lake water collected in October 2018 and 12-72 days in that collected in May 2019. The biodegradation of BPs in summer was significantly higher than that in autumn. The presence of HA promoted the disappearance of BPs from Taihu Lake water by adsorbing and binding BPs. The disappearance rate of BPs accelerated with increasing concentrations of HA. However, the presence of HA decreased the biodegradation of BPs. When the concentration of HA was 10 mg/L, the single-adsorption capacities for BPS, BPA, BPB, BPM and BPAF were 3.18-10.33 mg/g in the Taihu Lake water with little desorption. BP adsorption and desorption in the BP mixtures were different from that in the single BPs. Competitive desorption occurred among the mixtures. The results of this study are the first to indicate the biodegradation of eight BPs in natural lake water and the possible effect of HA on the fate of BPs in the environment.

Bioaccumulation, physiological distribution, and biotransformation of tetrabromobisphenol a (TBBPA) in the geophagous earthworm Metaphire guillelmi - hint for detoxification strategy.

The mechanisms underlying the bioaccumulation and detoxification of tetrabromobisphenol A (TBBPA) by terrestrial invertebrates are poorly understood. We used uniformly ring-14C-labelled TBBPA to investigate the bioaccumulation kinetics, metabolites distribution, and subsequent detoxification strategy of TBBPA in the geophagous earthworm Metaphire guillelmi in soil. The modeling of bioaccumulation kinetics showed a higher biota-soil-accumulation-factor of total 14C than that of the parent compound TBBPA, indicating that most of the ingested TBBPA was transformed into metabolites or sequestered as bound residues in the earthworms. Bound-residue formation in the digestive tract may hinder the accumulation of TBBPA in other parts of the body. Nonetheless, via the circulatory system, TBBPA was transferred to other tissues, especially the clitellum region, where sensitive organs are located. In the clitellum region, TBBPA was quickly transformed to less toxic dimethyl TBBPA ether and rapidly depurated through feces. We conclude that the detoxification of TBBPA in M. guillelmi occurred via bound-residue formation in the digestive tract as well as the generation and depuration of O-methylation metabolites. Our results provided direct evidence of TBBPA detoxification in earthworms. Further researches are needed to confirm whether O-methylation coupled with depuration is a common detoxification strategy for phenolic xenobiotics in other soil organisms needs to be determined.

siPRDX2-elevated DNM3 inhibits the proliferation and metastasis of colon cancer cells via AKT signaling pathway.

Purpose: We have previously reported that PRDX2 plays an oncogenic role in colon cancer. In this study, the mRNA expression profile of PRDX2 in HCT116 cells was investigated. Furthermore, we selected Dynamin 3 (DNM3), which is up-regulated by siPRDX2, to investigate its expression pattern and functions in colon cancer. Patients and methods: PRDX2 siRNA was transfected into HCT116 cells and the mRNA profile was tested by RNA-Sequencing. The expression of interest proteins was determined by Western blot. DNM3 expression in colon cancer tissues and para-carcinoma tissues was evaluated by Western blot and immunohistochemistry assays. Full-length cDNA of DNM3 was cloned into pcDNA3.1 and introduced into HCT116 and HT29 cells. Cell proliferation was tested by CCK-8 and colony formation assays. Cell invasion and migration were tested by transwell assays. Gelatin zymography was utilized for detection of MMP9 activity. Cell apoptosis was investigated with Annexin V/PI staining and flow cytometry and visualized with Hoechst/PI staining assay. All statistical analysis was performed with SPSS 17.0 software. Results: PRDX2 knockdown led to 210 up-regulated genes and 16 down-regulated genes in HCT116 cells. We also found that DNM3 expression was up-regulated following PRDX2 silencing in HCT116 and HT29 cells. In colon cancer patients, DNM3 was down-regulated and showed a significant association with pathologic grading. DNM3 overexpression inhibited cell proliferation and induced apoptosis in HCT116 and HT29 cells. Cell migration and invasion were also down-regulated in DNM3 overexpressing colon cancer cells, which might be due to the inhibition of MMP9 proteolytic activities. After thorough investigation of the potential mechanism involved, we hypothesized that DNM3 overexpression induced activation of the mitochondrial apoptosis pathway and inhibition of the AKT pathway. Conclusion: These data suggest that DNM3 is down-regulated in colon cancer, serving as a tumor suppressor. Our study provides new sights into the prognostic value and therapeutic application of DNM3 in colon cancer.

Aging Significantly Affects Mobility and Contaminant-Mobilizing Ability of Nanoplastics in Saturated Loamy Sand.

Plastic debris, in particular, microplastics and nanoplastics, is becoming an emerging class of pollutants of global concern. Aging can significantly affect the physicochemical properties of plastics, and therefore, may influence the fate, transport, and effects of these materials. Here, we show that aging by UV or O3 exposure drastically enhanced the mobility and contaminant-mobilizing ability of spherical polystyrene nanoplastics (PSNPs, 487.3 ± 18.3 nm in diameter) in saturated loamy sand. Extended Derjaguin-Landau-Verwey-Overbeek calculations and pH-dependent transport experiments demonstrated that the greater mobility of the aged PSNPs was mainly the result of surface oxidation of the nanoplastics, which increased not only the surface charge negativity, but more importantly, hydrophilicity of the materials. The increased mobility of the aged PSNPs significantly contributed to their elevated contaminant-mobilizing abilities. Moreover, aging of PSNPs enhanced the binding of both nonpolar and polar contaminants, further increasing the contaminant-mobilizing ability of PSNPs. Interestingly, aging enhanced binding of nonpolar versus polar compounds via distinctly different mechanisms: increased binding of nonpolar contaminants (tested using pyrene) was mainly the result of the modification of the polymeric structure of PSNPs that exacerbated slow desorption kinetics; for polar compounds (4-nonylphenol), aging induced changes in surface properties also resulted in irreversible adsorption of contaminants through polar interactions, such as hydrogen bonding. The findings further underline the significant effects of aging on environmental fate and implications of nanoplastics.

The relevance of MTHFR C677T, A1298C, and MTRR A66G polymorphisms with response to male infertility in Asians: A meta-analysis.

Although published studies have reported the association between MTHFR C677T (rs 1801133), A1298C (rs 1801131), and MTRR A66G (rs1801394) polymorphisms and male infertility in Asian populations, the results are conflicting. In order to accurately evaluate the relevance, a meta-analysis was performed.We searched for potential studies in 4 databases, containing PubMed, ScienceDirect, China National Knowledge Infrastructure (CNKI), and Wanfang database until May 31, 2018. The summarized odds ratio (OR) with 95% confidence intervals (95% CI) were calculated to evaluate the relevance in 5 genetic models. The heterogeneity test, sensitivity analysis, and publication bias test was performed by Review Manager 5.3 software.Overall, 22 case-control studies with 5049 cases and 4157 controls were included in this meta-analysis, which contained 20 studies of MTHFR C677T polymorphism, 12 studies of MTHFR A1298C polymorphism and 4 studies of MTRR A66G polymorphism. The results indicated that MTHFR C677T, A1298C, and MTRR A66G polymorphisms were significantly associated with male infertility in Asian populations (Dominant model: MTHFR CC + CT vs TT: OR = 0.60, 95% CI (0.53, 0.67), P <.00001; MTHFR AA + AC vs CC: OR = 0.62, 95% CI (0.49, 0.79), P = .0001; MTRR AA + AG vs GG: OR = 0.60, 95% CI (0.45, 0.81), P = .001. Recessive model: MTHFR CC vs CT + TT: OR = 0.67, 95% CI (0.61, 0.74), P <.00001; MTHFR AA vs AC + CC: OR = 0.79, 95% CI (0.70, 0.88), P <.0001; MTRR AA vs AG + GG: OR = 0.70, 95% CI (0.56, 0.88), P = .002. Heterozygote model: MTHFR CC vs CT: OR = 0.74, 95% CI (0.67, 0.82), P <.00001; MTHFR AA vs AC: OR = 0.83, 95% CI (0.73, 0.93), P = .002; MTRR AA vs AG: OR = 0.76, 95% CI (0.60, 0.92), P = .02. Homozygote model: MTHFR CC vs TT: OR = 0.48, 95% CI (0.41, 0.56), P <.00001; MTHFR AA vs CC: OR = 0.61, 95% CI (0.39, 0.93), P = .02; MTRR AA vs GG: OR = 0.51, 95% CI (0.36, 0.72), P = .0001. Allele model: MTHFR C vs T: OR = 0.70, 95% CI (0.66, 0.75), P <.00001; MTHFR A vsC: OR = 0.82, 95% CI (0.71, 0.95), P = .01; MTRR A vs G: OR = 0.76, 95% CI (0.66, 0.88), P = .00003). Stratified analyses by geographical location and source of controls showed the same results. Sensitivity analyses indicated that the final consequences of this meta-analysis were stable, and the publication biases test had not found obvious asymmetry.This meta-analysis indicates that MTHFR C677T, A1298C, and MTRR A66G polymorphisms are the risk factors with susceptibility to male infertility in Asians.

Quantifying the bioaccumulation of nanoplastics and PAHs in the clamworm Perinereis aibuhitensis.

The impact of nanometer-scale plastics (<1000 nm nanoplastics, NPs) on the bioaccumulation of hydrophobic organic pollutants, and especially polycyclic aromatic hydrocarbons (PAHs), in marine organisms has become of urgent concern. However, simultaneous determinations of the bioaccumulation of NPs and PAHs have been hindered by the lack of an efficient digestion method that removes background interference from the tissue without altering the surface properties of the plastic and destroying the PAHs. To solve this problem, an enzymatic digestion-based protocol using proteinase K and subsequent quantification methods were developed on a typical marine benthic invertebrate - the clamworm Perinereis aibuhitensis. Enzymatic digestion removed 91% of the biological tissues, comparable to the amount removed using 65% HNO3 (93% removed) and better than that removed using 30% H2O2 or 10% KOH digestion (76% and 66%, respectively). After enzymatic digestion, roughly 92% of the NPs and 88% of the amount of pyrene were recovered, without significant modification of the NPs or pyrene degradation. By contrast, the NP and pyrene recovery achieved with HNO3 digestion was only 1.4% and 0.1%, respectively. The newly developed protocol was successfully applied to a 96-h bioaccumulation study. The use of radioactively labeled 14C-pyrene and fluorescently labeled NPs allowed the simultaneous quantification of NPs and PAHs in the clamworm and revealed a bioconcentration factor (BCF) of 1.96 ±â€¯0.93 and 402.7 ±â€¯47.0, respectively. The quantification of NPs and pyrene indicated that NP-adsorbed pyrene accounted for <1% of the total pyrene accumulation in the clamworm body when the concentration of NPs in seawater was as low as 0.4 mg/L. Our enzymatic digestion and dual-labeling technique thus provides the first reported BCF value of NPs in a marine benthic organism and new insights into the vector effects of these particles on the bioaccumulation of organic contaminants in a marine ecosystem.

A multi-jointed underactuated robot hand with fluid-driven stretchable tubes.

Inspired from flexible bending of octopus' tentacles and outside-driving kind of traditional exoskeletons, this paper proposed a novel self-adaptive underactuated finger mechanism, called OS finger. OS finger is similar to an octopus' tentacle and consists of an artificial muscle which is through all joints and driven by fluid, eight serial-hinged joints, and force-changeable assembly. The force-changeable assembly is mainly composed of a spring and elastic rubber membrane, which is coordinated for stable grasping by a layer of rubber material in the surface of the finger. OS finger can execute different grasping modes depending on the shapes and dimensions of the grasped objects and grip objects in a gentle and form-fitting manner. The OS finger combines good qualities of both rigid grasp of traditional fingers and form-fitting grasp of flexible fingers. Kinematic analysis and experimental results show that the OS robot Hand with four OS fingers is valid for precise pinching, self-adaptive powerful encompassing, and grasping forces that are freely changeable in a wide range. With the advantage of high self-adaptation, various grasp configurations and large range of grasping forces, the OS Hand has a wide range of applications in the area of service robotics which requires a lot of flexible operations of general grasping, moving and releasing.